Fields were photographed with a SPOT-RT digital camera and acquisition software (Diagnostic Instruments, Sterling Heights, MI). Drew University of Medicine and Science, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California 90095Department of Internal Medicine (L. The merge image was obtained by fusing the green (FITC) and the blue (DAPI) filters pictures. Drew University of Medicine and Science, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California 90095Department of Internal Medicine (L. This was done by investigating the expression of key pro- and anti-cell differentiation lineage markers and select growth factors modulated by 1,25-D3 in a well-known and widely used skeletal muscle cell line model.
Vitamin D, a fat-soluble secosteroid prohormone, is obtained from sun exposure or from dietary sources.Have a browse of our current deals below and take advantage of these great reasons to book online with us.From our great value Advance tickets booked up to 12 weeks ahead, to our special offers - start planning your next train journey and get saving today. RT Profiler PCR pathway focused arrays (SABiosciences Corp., Frederick, MD) were applied in triplicate to detect changes in gene expression of skeletal muscle growth and differentiation factors. Negative controls were done by either omitting the first antibody or using a rabbit nonspecific Ig G (18).
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The nonspecific binding sites/epitopes were blocked with 10% normal goat serum and incubated with an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C terminus of VDR at a dilution of (Santa Cruz Biotechnology, Inc., Santa Cruz, CA).